Download Biophysical Methods for Biotherapeutics: Discovery and by Tapan K. Das PDF

Analytic

By Tapan K. Das

ISBN-10: 0470938439

ISBN-13: 9780470938430

With a spotlight on sensible functions of biophysical suggestions, this publication hyperlinks basic biophysics to the method of biopharmaceutical development.

• Helps formula and analytical scientists in pharma and biotech larger comprehend and use biophysical equipment
• Chapters geared up in accordance with the sequential nature of the drug improvement strategy
• Helps formula, analytical, and bioanalytical scientists in pharma and biotech greater comprehend and usestrengths and boundaries of biophysical equipment
• Explains tips to use biophysical tools, the data bought, and what should be provided in a regulatory submitting, verify influence on caliber and immunogenicity
• With a spotlight on sensible purposes of biophysical concepts, this e-book hyperlinks primary biophysics to the method of biopharmaceutical development.

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Additional info for Biophysical Methods for Biotherapeutics: Discovery and Development Applications

Sample text

These intensity data are actually only half of the data necessary to reconstruct the electron density and only provides information on the vectors between atoms [67] but none on the positions of atoms relative to an origin. To derive the positional electron density information in real space a Fourier transform of the structure factors of the reflections must be performed. The structure factor consists of a vector of amplitude given by the square root of the intensity and angle called the phase of a reflection.

Knowing these, the location of every reflection on the images can be computed and raw images can be processed by integrating a spot to reduce to a three-dimensional integer coordinate with an associated value and percentage of the recording reflection that was on the image. Finally, reflections are scaled together to yield a data set of intensities by merging like reflections, bringing them onto a common scale. This process yields statistics given in resolution shells from low to high resolution.

Biosensor data for anti-IGF1R Adnectin binding to IGF1R-Fc from different species: human (a), monkey (b), mouse (c and d) and rat (e and f) IGF1R. In each panel, the IGF1R-Fc target was captured on the surface by protein A and the Adnectin was flowed across the surface at multiple concentrations from 1 to 500 nM. The data for human and monkey IGF1R were well described by a simple Langmuir fitting model. In contrast, the kinetic data for mouse and rat (c, e) could not be well described with a simple model.

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