Download Apoptosis Methods in Pharmacology and Toxicology: Approaches by Myrtle A. Davis PDF


By Myrtle A. Davis

ISBN-10: 0896038904

ISBN-13: 9780896038905

Univ. of Maryland, Baltimore. Discusses laser scanning cytometry for the research of apoptosis, tissue-based tools of quantification for stories utilizing animal types, and sensible assays and ELISA recommendations. DNLM: Apoptosis--physiology.

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1997) Movement of Bax from the cytosol to mitochondria during apoptosis. J. Cell Biol. 139(5), 1281–1292. Mahajan, N. , Gordon, G. , and Herman, B. (1998) Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer [see comments]. Nat. Biotechnol. 16(6), 547–552. Qin, Z. , Kikly, K. , Kegel, K. , and DiFiglia, M. (2000) Pro-caspase-8 is Predominatly localized in mitochondria and released into cytoplasm upon apoptotic stimulation. J.

2(10), 754–761. Petit, J. , Maftah, A, Ratinaud, M. , and Julien, R. (1992) 10N-nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria. Eur. J. Biochem. 209(1), 267–273. , Zweier, J. , Kinzler, K. , and Vogelstein, B. (1997). A model for p53-induced apoptosis [see comments]. Nature 389(6648), 300–305. Pierce, R. , Campbell, J. , Stephenson, A. , Franklin, C. , et al. (2000) Disruption of redox homeostasis in tumor necrosis factor-induced apoptosis in a murine hepatocyte cell line.

Dye solutions decompose rapidly if exposed to light. 11. Dye concentrations in the range of 100–200 nM are recommended, because at higher concentrations nonmitochondrial staining may occur. 12. Cells stained with MitoTracker Green FM show maximal emission at 516 nm; cells stained with CMXRosamine show maximal absorption at 594 nm and emit maximally at 608 nm; they also exhibit significant absorption in the UV region of the spectrum and may be excitable with a mercury arc lamp. During the staining period cells tend to clump; to obtain meaningful data on a per cellbasis, it is essential to resuspend cells by briefly and gently vortexing immediately before analysis.

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